Recent Publications

Banerjee A, Hamberger B. “P450s controlling metabolic bifurcations in plant terpene specialized metabolism.” Phytochem Rev. (2018) 17(1):81-111. doi: 10.1007/s11101-017-9530-4

Heskes AM, Sundram TCM, Boughton BA, Jensen NB, Hansen NL, Crocoll C, Cozzi F, Rasmussen S, Hamberger B, Hamberger B, Staerk D, Møller BL, Pateraki I. “Biosynthesis of bioactive diterpenoids in the medicinal plant Vitex agnus-castus.” Plant J. (2018) Mar;93(5):943-958. doi: 10.1111/tpj.13822

Forman V, Callari R, Folly C, Heider H, Hamberger B. “Production of Putative Diterpene Carboxylic Acid Intermediates of Triptolide in Yeast.” Molecules. (2017) Jun 13;22(6). pii: E981. doi: 10.3390/molecules22060981

Pateraki I, Andersen-Ranberg J, Jensen NB, Wubshet SG, Heskes AM, Forman V, Hallström B, Hamberger B, Motawia MS, Olsen CE, Staerk D, Hansen J, Møller BL, Hamberger B. “Total biosynthesis of the cyclic AMP booster forskolin from Coleus forskohlii.” Elife. (2017) Mar 14;6. pii: e23001. doi: 10.7554/eLife.23001

Hansen NL, Nissen JN, Hamberger B. “Two residues determine the product profile of the class II diterpene synthases TPS14 and TPS21 of Tripterygium wilfordii.” Phytochemistry. (2017) Mar 6. pii: S0031-9422(17)30093-6. doi: j.phytochem.2017.02.022

Hansen NL, Heskes AM, Hamberger B, Olsen CE, Hallström BM, Andersen-Ranberg J, Hamberger B. “The terpene synthase gene family in Tripterygium wilfordii harbors a labdane-type diterpene synthase among the monoterpene synthase TPS-b subfamily.” Plant J. (2017) Feb;89(3):429-441. doi: 10.1111/tpj.13410

Laursen T, Borch J, Knudsen C, Bavishi K, Torta F, Martens HJ, Silvestro D, Hatzakis NS, Wenk MR, Dafforn TR, Olsen CE, Motawia MS, Hamberger B, Møller BL, Bassard JE. “Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum.” Science. (2016) Nov 18;354(6314):890-893. doi: 10.1126/science.aag2347

Jagalski V, Barker R, Topgaard D, Günther-Pomorski T, Hamberger B, Cárdenas M. “Biophysical study of resin acid effects on phospholipid membrane structure and properties.” Biochim Biophys Acta. (2016) Nov;1858(11):2827-2838. doi: 10.1016/j.bbamem.2016.08.008

Luo D, Callari R, Hamberger B, Wubshet SG, Nielsen MT, Anderson-Randberg J, Hallstrom BM, Cozzi F, Heider H, Lindberg Moller B, Staerk D, Hamberger B. “Oxidation and cyclization of casbene in the biosynthesis of Euphorbia factors from mature seeds of Euphorbia lanthyris L.“ PNAS (2016) Jul 6. doi:10.1073/pnas.1607504113

King BC, Vavitsas K, Ikram NK, Schrøder J, Scharff LB, Hamberger B, Jensen PE, Simonsen HT. “In vivo assembly of DNA-fragments in the moss, Physcomitrella patens.” Scientific Reports (2016) Apr 29;6:25030 doi:10.1038/srep25030

Wubshet SG, Tahtah Y, Heskes AM, Kongstad KT, Pateraki I, Hamberger B, Møller BL, Staerk D. “Identification of PTP1B and α-Glucosidase Inhibitory Serrulatanes from Eremophila spp. by Combined use of Dual High-Resolution PTP1B and α±-Glucosidase Inhibition Profiling and HPLC-HRMS-SPE-NMR.” J Nat Prod. (2016) Apr 22;79(4):1063-72. doi:10.1021/acs.jnatprod.5b01128

Andersen-Ranberg J, Kongstad KT, Nielsen MT, Jensen NB, Pateraki I, Bach SS, Hamberger B, Zerbe P, Staerk D, Bohlmann J, Møller BL, Hamberger B. “Expanding the Landscape of Diterpene Structural Diversity through Stereochemically Controlled Combinatorial Biosynthesis.” Angew Chem Int Ed Engl. (2016) Feb;55(6):2142-6. doi:10.1002/anie.201510650

Gnanasekaran T, Vavitsas K, Andersen-Ranberg J, Nielsen AZ, Olsen CE, Hamberger B, Jensen PE. “Heterologous expression of the isopimaric acid pathway in Nicotiana benthamiana and the effect of N-terminal modifications of the involved cytochrome P450 enzyme.” J Biol Eng. (2015) 9:24. doi:10.1186/s13036-015-0022-z

Englund E, Andersen-Ranberg J, Miao R, Hamberger B, Lindberg P. “Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide.” ACS Synth Biol. (2015) Dec 18;4(12):1270-8. doi:10.1021/acssynbio.5b00070

Patron NJ, Orzaez D, Marillonnet S, Warzecha H, Matthewman C, Youles M, Raitskin O, Leveau A, Farré G, Rogers C, Smith A, Hibberd J, Webb AA, Locke J, Schornack S, Ajioka J, Baulcombe DC, Zipfel C, Kamoun S, Jones JD, Kuhn H, Robatzek S, Van Esse HP, Sanders D, Oldroyd G, Martin C, Field R, O'Connor S, Fox S, Wulff B, Miller B, Breakspear A, Radhakrishnan G, Delaux PM, Loqué D, Granell A, Tissier A, Shih P, Brutnell TP, Quick WP, Rischer H, Fraser PD, Aharoni A, Raines C, South PF, Ané JM, Hamberger BR, Langdale J, Stougaard J, Bouwmeester H, Udvardi M, Murray JA, Ntoukakis V, Schäfer P, Denby K, Edwards KJ, Osbourn A, Haseloff J. “Standards for plant synthetic biology: a common syntax for exchange of DNA parts.” New Phytol. (2015) Oct;208(1):13-9. doi:10.1111/nph.13532

Pateraki I, Heskes AM, Hamberger B. “Cytochromes P450 for terpene functionalisation and metabolic engineering.” Adv Biochem Eng Biotechnol. (2015) 148:107-39. doi:10.1007/10_2014_301

Zedler JAZ, Gangl D, Hamberger B, Purton S, Robinson C. “Stable Expression of a Bifunctional Diterpene Synthase in the Chloroplast of Chlamydomonas reinhardtii.” Journal of Applied Phycology (2014) doi:10.1007/s10811-014-0504-2

Nielsen MT, Ranberg JA, Christensen U, Christensen HB, Harrison SJ, Olsen CE, Hamberger B, Møller BL, Nørholm MH. “Microbial Synthesis of the Forskolin Precursor Manoyl Oxide in an Enantiomerically Pure Form.” Appl Environ Microbiol. (2014) Dec;80(23):7258-65. doi:10.1128/AEM.02301-14

Zerbe P, Chiang A, Dullat H, O'Neil-Johnson M, Starks C, Hamberger B, Bohlmann J. “Diterpene synthases of the biosynthetic system of medicinally active diterpenoids in Marrubium vulgare.” Plant J. (2014) Sep;79(6):914-27. doi:10.1111/tpj.12589

Pateraki I, Andersen-Ranberg J, Hamberger B, Heskes AM, Martens HJ, Zerbe P, Bach SS, Møller BL, Bohlmann J, Hamberger B. “Manoyl oxide (13R), the biosynthetic precursor of forskolin, is synthesized in specialized root cork cells in Coleus forskohlii.” Plant Physiol. (2014) Mar;164(3):1222-36. doi:10.1104/pp.113.228429

Renault H, Bassard JE, Hamberger B, Werck-Reichhart D. “Cytochrome P450-mediated metabolic engineering: current progress and future challenges.” Curr Opin Plant Biol. (2014) Jun;19:27-34. doi:10.1016/j.pbi.2014.03.004

Bach SS, Bassard JÉ, Andersen-Ranberg J, Møldrup ME, Simonsen HT, Hamberger B. “High-throughput testing of terpenoid biosynthesis candidate genes using transient expression in Nicotiana benthamiana.” Methods Mol Biol. 2014;1153:245-55. doi: 10.1007/978-1-4939-0606-2_18

Bach SS, King BC, Zhan X, Simonsen HT, Hamberger B. “Heterologous stable expression of terpenoid biosynthetic genes using the moss Physcomitrella patens.” Methods Mol Biol. (2014) 1153:257-71. doi:10.1007/978-1-4939-0606-2_19

Hamberger B, Bak S. “Plant P450s as versatile drivers for evolution of species-specific chemical diversity.” Philos Trans R Soc Lond B Biol Sci. (2013) Feb 19;368(1612):20120426. doi:10.1098/rstb.2012.0426

Zerbe P, Hamberger B, Yuen MM, Chiang A, Sandhu HK, Madilao LL, Nguyen A, Hamberger B, Bach SS, Bohlmann J. “Gene discovery of modular diterpene metabolism in nonmodel systems.” Plant Physiol. (2013) Jun;162(2):1073-91. doi:10.1104/pp.113.218347

Zerbe P, Chiang A, Yuen M, Hamberger B, Hamberger B, Draper JA, Britton R, Bohlmann J. “Bifunctional cis-abienol synthase from Abies balsamea discovered by transcriptome sequencing and its implications for diterpenoid fragrance production.” J Biol Chem. (2012) Apr 6;287(15):12121-31. doi:10.1074/jbc.M111.317669

Bak S, Beisson F, Bishop G, Hamberger B, Höfer R, Paquette S, Werck-Reichhart D. “Cytochromes p450.” Arabidopsis Book. (2011) 9:e0144. doi:10.1199/tab.0144

Hamberger B, Ohnishi T, Hamberger B, Séguin A, Bohlmann J. “Evolution of diterpene metabolism: Sitka spruce CYP720B4 catalyzes multiple oxidations in resin acid biosynthesis of conifer defense against insects.” Plant Physiol. (2011) Dec;157(4):1677-95. doi:10.1104/pp.111.185843

Porth I, Hamberger B, White R, Ritland K. “Defense mechanisms against herbivory in Picea: sequence evolution and expression regulation of gene family members in the phenylpropanoid pathway.” BMC Genomics. (2011) Dec 16;12:608. doi:10.1186/1471-2164-12-608

Kaneda M, Schuetz M, Lin BS, Chanis C, Hamberger B, Western TL, Ehlting J, Samuels AL. “ABC transporters coordinately expressed during lignification of Arabidopsis stems include a set of ABCBs associated with auxin transport.” J Exp Bot. (2011) Mar;62(6):2063-77. doi:10.1093/jxb/erq416

Abbott E, Hall D, Hamberger B, Bohlmann J. “Laser microdissection of conifer stem tissues: isolation and analysis of high quality RNA, terpene synthase enzyme activity and terpenoid metabolites from resin ducts and cambial zone tissue of white spruce (Picea glauca).” BMC Plant Biol. (2010) Jun 12;10:106. doi:10.1186/1471-2229-10-106

Hamberger B, Hall D, Yuen M, Oddy C, Hamberger B, Keeling CI, Ritland C, Ritland K, Bohlmann J. “Targeted isolation, sequence assembly and characterization of two white spruce (Picea glauca) BAC clones for terpenoid synthase and cytochrome P450 genes involved in conifer defence reveal insights into a conifer genome.” BMC Plant Biol. (2009) Aug 6;9:106. doi:10.1186/1471-2229-9-106

Kim JE, Cheng KM, Craft NE, Hamberger B, Douglas CJ. “Over-expression of Arabidopsis thaliana carotenoid hydroxylases individually and in combination with a beta-carotene ketolase provides insight into in vivo functions.” Phytochemistry. (2010) Feb;71(2-3):168-78. doi:10.1016/j.phytochem.2009.10.011

Souza Cde A, Barbazuk B, Ralph SG, Bohlmann J, Hamberger B, Douglas CJ. “Genome-wide analysis of a land plant-specific acyl:coenzyme A synthetase (ACS) gene family in Arabidopsis, poplar, rice and Physcomitrella.” New Phytol. (2008) 179(4):987-1003. doi:10.1111/j.1469-8137.2008.02534.x

Ehlting J, Hamberger B, Ginglinger JF, Werck-Reichhart D. “Genome Wide Approaches in Natural Product Research” in: A.E. Osbourn and V. Lanzotti (eds.), Plant-derived Natural Products. Invited review. (2009)

Hamberger B, Ellis M, deAzevedo Souza C, Barbazuk B, Douglas CJ. “Genome-wide analyses of phenylpropanoid gene families and superfamilies in Populus trichocarpa, Arabidopsis thaliana, and Oryza sativa: the Populus lignification toolbox and diversification angiosperm gene functions.” Canadian Journal of Botany (2007) 85(12): 1182–1201.

Hamberger B, Ehlting J, Barbazuk B, Douglas CJ. “Comparative genomics of the shikimate pathway in Arabidopsis, Populus trichocarpa and Oryza sativa: shikimate pathway gene family structure and identification of candidates for missing links in phenylalanine biosynthesis.” In: Recent Advances in Phytochemistry. Volume 40. Integrative Plant Biochemistry (J. T. Romeo, ed.), Elsevier Ltd, Amsterdam. (2006)

Ehlting J, Hamberger B, Million-Rousseau R, Werck-Reichhart D. “Cytochromes P450 in phenolic metabolism.” Phytochemistry Reviews (2006) V5: 239-270. Invited review.

Soltani BM, Ehlting J, Hamberger B, Douglas CJ. “Multiple cis-regulatory elements regulate distinct and complex patterns of developmental and wound-induced expression of Arabidopsis thaliana 4CL gene family members.” Planta. (2006) Oct;224(5):1226-38. doi:10.1007/s00425-006-0296-y

Hamberger B, Bohlmann J. “Cytochrome P450 mono-oxygenases in conifer genomes: discovery of members of the terpenoid oxygenase superfamily in spruce and pine.” Biochem Soc Trans. (2006) Dec;34(Pt 6):1209-14. doi:10.1042/BST0341209

Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, Déjardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasjärvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Leplé JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouzé P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D. “The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).” Science. (2006) Sep 15;313(5793):1596-604. doi:10.1126/science.1128691

Gilchrist EJ, Haughn GW, Ying CC, Otto SP, Zhuang J, Cheung D, Hamberger B, Aboutorabi F, Kalynyak T, Johnson L, Bohlmann J, Ellis BE, Douglas CJ, Cronk QC. “Use of Ecotilling as an efficient SNP discovery tool to survey genetic variation in wild populations of Populus trichocarpa.” Mol Ecol. (2006) Apr;15(5):1367-78. doi:10.1111/j.1365-294X.2006.02885.x

Schneider K, Hovel K, Witzel K, Hamberger B, Schomburg D, Kombrink E, Stuible HP. “The substrate specificity-determining amino acid code of 4-Coumarate:CoA ligase.” PNAS USA (2003) 100(14):8601-6.

Hahlbrock K, Bednarek P, Ciolkowski I, Hamberger B, Heise A, Liedgens H, Logemann E, Nürnberger T, Schmelzer E, Somssich IE, Tan JW. “Non-self recognition, transcriptional reprogramming, and secondary metabolite accumulation during plant/pathogen interactions.” PNAS USA (2003) 100 Suppl 2:14569-76.

Bednarek P, Winter J, Hamberger B, Oldham N J, Schneider B, Tan JW, Hahlbrock K. “Induction of 3'-O-β-d-ribofuranosyl adenosine during compatible, but not during incompatible, interactions of Arabidopsis thaliana or Lycopersicon esculentum with Pseudomonas syringae pathovar tomato.” Planta. (2004) 218(4):668-72.

Hamberger B, Hahlbrock K. “The 4-coumarate:CoA ligase gene family in Arabidopsis thaliana comprises one rare, sinapate-activating and three commonly occurring isoenzymes.” Proc Natl Acad Sci U S A. (2004) Feb 17;101(7):2209-14. doi:10.1073/pnas.0307307101

Book Chapter

Pateraki I, Heskes AM, Hamberger B. “Cytochromes P450 for terpene functionalisation and metabolic engineering.” Adv Biochem Eng Biotechnol. (2015) 148:107-39. doi:10.1007/10_2014_301

Renault H, Bassard JE, Hamberger B, Werck-Reichhart D. “Cytochrome P450-mediated metabolic engineering: current progress and future challenges.” Curr Opin Plant Biol. (2014) Jun;19:27-34. doi:10.1016/j.pbi.2014.03.004

Hamberger B, Bak S. “Plant P450s as versatile drivers for evolution of species-specific chemical diversity.” Philos Trans R Soc Lond B Biol Sci. (2013) Feb 19;368(1612):20120426. doi:10.1098/rstb.2012.0426

Bak S, Beisson F, Bishop G, Hamberger B, Höfer R, Paquette S, Werck-Reichhart D. “Cytochromes p450.” Arabidopsis Book. (2011) 9:e0144. doi:10.1199/tab.0144

Ehlting J, Hamberger B, Ginglinger JF, Werck-Reichhart D. “Genome Wide Approaches in Natural Product Research” in: A.E. Osbourn and V. Lanzotti (eds.), Plant-derived Natural Products. Invited review. (2009)

Hamberger B, Ehlting J, Barbazuk B, Douglas CJ. “Comparative genomics of the shikimate pathway in Arabidopsis, Populus trichocarpa and Oryza sativa: shikimate pathway gene family structure and identification of candidates for missing links in phenylalanine biosynthesis.” In: Recent Advances in Phytochemistry. Volume 40. Integrative Plant Biochemistry (J. T. Romeo, ed.), Elsevier Ltd, Amsterdam. (2006)

Ehlting J, Hamberger B, Million-Rousseau R., Werck-Reichhart D. “Cytochromes P450 in phenolic metabolism.” Phytochemistry Reviews (2006) V5: 239-270. Invited review.