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Recent Publications

Chaturvedi, S.S., Ramanan, R., Hu, J., Hausinger, R.P., and Christov, C.Z. (2021) Atomic and electronic structure determinants distinguish between ethylene formation and L‑arginine hydroxylation reaction mechanisms in the ethylene-forming enzyme. ACS Catalysis. (in press)

Kuliyev, E.*, Zhang, C.*, Sui, D., and Hu, J. Zinc transporter mutations linked to Acrodermatitis enteropathica disrupt function and cause mistrafficking. (2021) J Biol Chem (in press) (*Contributed equally to this study)

Hu, J. (2020) Toward unzipping the ZIP metal transporters: structure, evolution, and implications on drug discovery against cancer. FEBS J. (in press, invited State-of-the-Art Review)

Nevarez, J., Turmo, A., Hu, J., and Hausinger, R.P. (2020) Biological pincer complexes. ChemCatChem, 12(17):4242-4254. PMID: 33072225

Desguin, B., Urdiain-Arraiza, J., Costa, M., Fellner, M., Hu, J., Hausinger, R.P., Desmet, T., Hols, P., and Soumillion, P. (2020) Uncovering a superfamily of nickel-dependent hydroxyacid racemases and epimerases. Sci Rep, 10(1):18123. PMID: 33093595

Campeciño, J., Lagishetty, S., Wawrzak, Z., Alfaro, V.S., Lehnert, N., Reguera, G., Hu, J. and Hegg, E. (2020) Cytochrome c nitrite reductase from Geobacter lovleyi represents a new NrfA subclass. J of Biol Chem, 295(33):11455-11465. PMID: 32518164

Ding. D., Dong, K., Liu, J., Wei, C., Stanton, A., Min, J., Hu, J. and Chen, C. (2020) LOTUS domain is a novel class of G-quaduplex RNA binding domain. Nucleic Acids Res, 48(16):9262-9272. PMID: 32766792

Deng, X., Xin, Y., Miller, C.L., Hamelberg, D., Kirberger, M., Moremen, K.W., Hu, J. and Yang, J.J. (2020) Structural Mechanism of Cooperative Regulation of Calcium Sensing Receptor Mediated Cellular Signaling. Current Opinion in Physiology, 17: 269-277.

Good, N.M., Fellner, M., Demirer, K., Hu, J., Hausinger, R.P., Martinez-Gomez, N.C. (2020) Lanthanide-dependent alcohol dehydrogenases require an essential aspartate residue for metal coordination and enzymatic function. J Bio Chem, 295(24):8272-8284. PMID: 32366463

Zhang, C., Sui, D., Zhang, T. and Hu, J. (2020) Molecular basis of zinc-dependent endocytosis of human ZIP4 transceptor. Cell Rep, 31(4):107582. PMID: 32348750.

Fellner, M., Huizenga, K.G., Hausinger, R.P.*, Hu, J.* (2020) Crystallographic characterization of a tri-Asp metal-binding site at the three-fold symmetry axis of LarE. Sci Rep, 2020 Apr 2;10(1):5830 (*corresponding author) PMID: 32242052

Zhang, T., Sui, D., Zhang, C., Cole, L. and Hu, J. (2020) Asymmetric functions of a binuclear metal center within the transport pathway of a human zinc transporter ZIP4. The FASEB J, 34(1):237-247. PMID: 31914589

Zhang T, Kuliyev E, Sui D, Hu J. “The Histidine-rich Loop in the Extracellular Domain of ZIP4 Binds Zinc and Plays a Role in Zinc Transport.” Biochem J. (2019) Jun 4. doi: 10.1042/BCJ20190108

Fellner M, Hausinger RP, Hu J. “A structural perspective on the PP-loop ATP pyrophosphatase family.” Crit Rev Biochem Mol Biol. (2018) Oct 3;1-16. doi: 10.1080/10409238.2018.1516728

Hausinger RP, Desguin B, Fellner M, Rankin JA, Hu J. “Nickel-pincer nucleotide cofactor.” Curr Opin Chem Biol. (2018) Jul 9;47:18-23. doi: 10.1016/j.cbpa.2018.06.019

Zeng X, Uyar A, Sui D, Donyapour N, Wu D, Dickson A, Hu J. “Structural Insights into Lethal Contractural Syndrome Type 3 (LCCS3) Caused by a Missense Mutation of PIP5Kγ.” Biochem J. (2018) Jun 29;. doi: 10.1042/BCJ20180326

Rankin JA, Mauban RC, Fellner M, Desguin B, McCracken J, Hu J, Varganov SA, Hausinger RP. “Lactate Racemase Nickel-Pincer Cofactor Operates by a Proton-Coupled Hydride Transfer Mechanism.” Biochemistry. (2018) Jun 12;57(23):3244-3251. doi: 10.1021/acs.biochem.8b00100

Desguin B, Fellner M, Riant O, Hu J, Hausinger R, Hols P, Soumillion P. “Biosynthesis of the nickel-pincer nucleotide cofactor of lactate racemase requires a CTP-dependent cyclometallase.” J Biol Chem. (2018) Jun 10. doi: 10.1074/jbc.RA118.003741

Ye Y, Bian W, Fu F, Hu J, Liu H. “Selenoprotein S inhibits inflammation-induced vascular smooth muscle cell calcification.” J Biol Inorg Chem. (2018) May 2. doi: 10.1007/s00775-018-1563-7

Hu, J., Wille, H., and Schmitt-Ulms, G. “The Evolutionary unZIPping of a Dimerization Motif-A Comparison of ZIP and PrP Architectures.” Pathogens. (2017) Dec 29;7(1):4. doi: 10.3390/pathogens7010004

Martinez S, Fellner M, Herr CQ, Ritchie A, Hu J, Hausinger RP. “Structures and Mechanisms of the Non-Heme Fe(II)- and 2-Oxoglutarate-Dependent Ethylene-Forming Enzyme: Substrate Binding Creates a Twist.” J Am Chem Soc. (2017) Aug 30;139(34):11980-11988. doi: 10.1021/jacs.7b06186

Zhang T, Liu J, Fellner M, Zhang C, Sui D, Hu J. “Crystal structures of a ZIP zinc transporter reveal a binuclear metal center in the transport pathway.” Sci Adv. (2017) Aug 25;3(8):e1700344. doi: 10.1126/sciadv.1700344

Fellner M, Desguin B, Hausinger RP, Hu J. “Structural insights into the catalytic mechanism of a sacrificial sulfur insertase of the N-type ATP pyrophosphatase family, LarE.” Proc Natl Acad Sci U S A. (2017) Aug 7. pii: 201704967. doi: 10.1073/pnas.1704967114

Desguin, B., Soumillion, P., Hols, P., Hu, J., Hausinger, R.P. “Lactate Racemase and Its Niacin-Derived, Covalently-Tethered, Nickel Cofactor.” The Biological Chemistry of Nickel (2017) Mar 220-236 (book chapter).

Liu A, Sui D, Wu D, Hu J. “The activation loop of PIP5K functions as a membrane sensor essential for lipid substrate processing.” Science Advances. (2016) Nov 18;2(11). doi: 10.1126/sciadv.1600925

Feature Story

Zhang C*, Zhang T*, Zou J*, Miller CL*, Gorkhali R, Yang J, Schilmiller A, Wang S, Huang K, Brown EM, Moreman KW, Hu J, Yang JJ. “Structural basis for regulation of human calcium-sensing receptor by magnesium ions and an unexpected tryptophan derivative co-agonist.” ScienceAdvances. (2016) May 27. 2:e1600241 (*Contributed equally to this study, Corresponding author) doi:10.1126/sciadv.1600241

Zhang T, Sui D, Hu J. “Structural insights of ZIP4 extracellular domain critical for optimal zinc transport.” Nat Commun. (2016) Jun 20;7:11979. doi:10.1038/ncomms11979

Hu J, Yuan Q, Kang X, Qin Y, Li L, Ha Y, Wu D. “Resolution of structure of PIP5K1A reveals molecular mechanism for its regulation by dimerization and dishevelled.” Nat Commun. (2015) Sep 14;6:8205. doi:10.1038/ncomms9205

Desguin B, Zhang T, Soumillion P, Hols P, Hu J*, Hausinger RP*. “METALLOPROTEINS. A tethered niacin-derived pincer complex with a nickel-carbon bond in lactate racemase.” Science. (2015) Jul 3;349(6243):66-9. (*Corresponding author) doi:10.1126/science.aab2272

Kuo IY, Hu J, Ha Y, Ehrlich BE. “Presenilin-like GxGD membrane proteases have dual roles as proteolytic enzymes and ion channels.” J Biol Chem., (2015) Mar 6;290(10):6419-27. doi:10.1074/jbc.M114.629584

Hu J, Xue Y, Lee S, Ha Y. “The crystal structure of GXGD membrane protease FlaK.” Nature. (2011) Jul 17;475(7357):528-31. doi:10.1038/nature10218.

Hu J, Qin H, Gao FP Cross TA. “A Systematic Assessment of Mature MBP in Membrane Protein Production: Overexpression, Membrane targeting and Purification.” Protein Expression & Purification. (2011) Nov;80(1):34-40. doi:10.1016/j.pep.2011.06.001

Lee S, Xue Y, Hu J, Wang Y, Liu X, Demeler B, Ha Y. “The E2 Domains of APP and APLP1 Share a Conserved Mode of Dimerization.” Biochemistry. (2011) Jun 21;50(24):5453-5464 doi:10.1021/bi101846x

Hu J, Sharma M, Qin H, Gao FP, Cross TA. “Ligands Binding on the Interhelical Loop of CorA, a Magnesium Transporter from Mycobacterium Tuberculosis.” Journal of Biological Chemistry (2009) Jun 5;284(23):15619-15628 doi:10.1074/jbc.M901581200

Qin H, Hu J, Hua Y, Challa SV, Cross TA, Gao FP. “Construction of a Series of Vectors for High Throughput Cloning and Expression Screening of Membrane Proteins from Mycobacterium tuberculosis.” BMC Biotechnology (2008) May 16;8(1):51 doi:10.1186/1472-6750-8-51

Hu J, Qin H, Sharma M, Cross TA, Gao FP. “Chemical Cleavage of Fusion Proteins for High level Production of Transmembrane Peptides and Protein Domains Containing Conserved Methionines.” Biochimica et Biophysica Acta - Biomembranes (2008) Apr;1778(4):1060-1066 doi:10.1016/j.bbamem.2007.12.024

Qin Y, Hu J, Yang X, Wang K. “Mastoparan/Mastoparan X Altered Binding Behavior of La3+ to Calmodulin in Ternary Complexes.” Journal of Inorganic Biochemistry. (2008) Feb;102(2):278-284. doi:10.1016/j.jinorgbio.2007.08.011

Page RC, Li C, Hu J, Gao FP, Cross TA. “Lipid Bilayers: An Essential Environment for the Understanding of Membrane Proteins.” Magnetic Resonance in Chemistry (2007) Dec;45 Suppl 1:S2-S11 doi:10.1002/mrc.2077