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Recent Publications

Li, W., D.B. Lybrand, F. Zhou, R.L. Last, E. Pichersky. “Pyrethrin biosynthesis: The cytochrome P450 oxidoreductase CYP82Q3 converts jasmolone to pyrethrolone.” Plant Physiol. 181:934–944. doi: 10.1104/pp.19.00499

Cao, P., S.J. Kim, A. Xing, C.A. Schenck, L. Liu, N. Jiang, J. Wang, R.L. Last and F. Brandizzi. 2019. “Homeostasis of branched-chain amino acids is critical for the activity of TOR signaling in Arabidopsis.” eLife 2019;8:e50747 doi: 10.7554/eLife.50747

Moore, B.M., P. Wang, P. Fan, A. Lee, B. Leong, Y.-R. Lou, C.A. Schenck, K. Sugimoto, R.L. Last, M.D. Lehti-Shiu, C. Barry, S.-H. Shiu. 2020. “Within and cross species predictions of plant specialized metabolism genes using transfer learning.” In Silico Plants. doi: 10.1093/insilicoplants/diaa005

Li W., D.B. Lybrand, H. Xu, F. Zhou, R.L. Last, E. Pichersky. 2020. A trichome-specific, plastid-localized Tanacetum cinerariifolium Nudix protein hydrolyzes the natural pyrethrin pesticide biosynthetic intermediate trans-chrysanthemyl diphosphate. Front. Plant Sci. 24 April 2020. doi: 10.3389/fpls.2020.00482

Alseekh, S., I. Offner, Z. Liu, S. Osorio, J.G. Vallarino, R.L. Last, D. Zamir, T. Toghe, A. Fernie. 2020. “Quantitative trait loci analysis (QTL) of seed specialized metabolites reveals seed-specific flavonols and differential regulation of glycoalkaloid content in tomato.” Plant J. doi 10.1111/tpj.14879

Lybrand D.B., X. Xu, R.L. Last, E. Pichersky. 2020. “How plants synthesize pyrethrins – safe and biodegradable insecticides.” Trends Plant Sci. doi 10.1016/j.tplants.2020.06.012.

Li, X., A.D. Jones, R.L. Last. 2020. “Switchgrass metabolomics reveals striking genotypic and developmental differences in saponins. BiorxivLink

Leong, B.J., S.M. Hurney, P.D. Fiesel, G.D. Moghe, A.D. Jones and R.L. Last. 2020. “Specialized metabolism in a non-model nightshade: trichome acylinositol biosynthesis.” Plant Physiol. 183: 915-924 Link

Fan, P., P. Wang, Y.-R. Lou, B.J. Leong, B.J. Moore, C.A. Schenck, R. Combs, P. Cao, F. Brandizzi, S.-H. Shiu and R.L. Last. 2020. “Evolution of a plant gene cluster in Solanaceae and emergence of metabolic diversity.” eLife. doi 10.7554/eLife.56717

Schenck CA, Last RL. “Location, Location! Cellular relocalization primes specialized metabolic diversification.” FEBS J. (2019) Oct 17;. doi: 10.1111/febs.15097

Li W, Lybrand DB, Zhou F, Last RL, Pichersky E. “Pyrethrin biosynthesis: The cytochrome P450 oxidoreducatse CYP82Q3 converts jasmolone to pyrethrolone.” Plant Physiol. (2019) 181:934–944. doi: 10.1104/pp.19.00499

Liu J, Lu Y, Hua W, Last RL. “A New Light on Photosystem II Maintenance in Oxygenic Photosynthesis.” Front Plant Sci. (2019) July 31;10:975. doi: 10.3389/fpls.2019.00975

Last RL. “Put on Your Sunscreen: The Birth of Arabidopsis Abiotic Stress Genetics.” Plant Cell. (2019) May 8;. doi: 10.1105/tpc.19.00341

Fan P, Leong BJ, Last RL. “Tip of the trichome: evolution of acylsugar metabolic diversity in Solanaceae.” Curr Opin Plant Biol. (2019) Apr 19;49:8-16. link

Leong BJ, Lybrand DB, Lou YR, Fan P, Schilmiller AL, Last RL. “Evolution of metabolic novelty: A trichome-expressed invertase creates specialized metabolic diversity in wild tomato.” Sci Adv. (2019) Apr;5(4):eaaw3754. doi: 10.1126/sciadv.aaw3754

Moore BM, Wang P, Fan P, Leong B, Schenck CA, Lloyd JP, Lehti-Shiu MD, Last RL, Pichersky E, Shiu SH. “Robust predictions of specialized metabolism genes through machine learning.” Proc Natl Acad Sci U S A. 2019 Jan 23. pii: 201817074. doi: 10.1073/pnas.1817074116

Xu H, Lybrand D, Bennewitz S, Tissier A, Last RL, Pichersky E. “Production of trans-chrysanthemic acid, the monoterpene acid moiety of natural pyrethrin insecticides, in tomato fruit.” Metab Eng. (2018) Apr 9. doi: 10.1016/j.ymben.2018.04.004

Fan P, Miller AM, Liu X, Jones AD, Last RL. “Evolution of a flipped pathway creates metabolic innovation in tomato trichomes through BAHD enzyme promiscuity.” Nat Commun. (2017) Dec 12;8(1):2080. doi: 10.1038/s41467-017-02045-7

Xu H, Moghe GD, Wiegert-Rininger K, Schilmiller AL, Barry CS, Last RL, Pichersky E. “Identification of dehydrogenases involved in the biosynthesis of the monoterpene moiety of pyrethrin.” Plant Physiol. (2017) Nov 9;. doi: 10.1104/pp.17.01330

Liu J, Last RL. “A chloroplast thylakoid lumen protein is required for proper photosynthetic acclimation of plants under fluctuating light environments.” Proc Natl Acad Sci U S A. (2017) Sep 5. doi: 10.1073/pnas.1712206114

Moghe GD, Leong BJ, Hurney S, Jones AD, Last RL. “Evolutionary routes to biochemical innovation revealed by integrative analysis of a plant-defense related specialized metabolic pathway.” Elife. (2017) Aug 30;. doi: 10.7554/eLife.28468

Leong BJ, Last RL. “Promiscuity, impersonation and accommodation: evolution of plant specialized metabolism.” Curr Opin Struct Biol. (2017) Aug 16;47:105-112. doi: 10.1016/

Xing A, Last RL. “A Regulatory Hierarchy of the Arabidopsis Branched-chain Amino Acid Metabolic Network.” Plant Cell. (2017) May 18. pii: tpc.00186.2017. doi: 10.1105/tpc.17.00186

Uygun S, Peng C, Lehti-Shiu MD, Last RL, Shiu SH. “Utility and Limitations of Using Gene Expression Data to Identify Functional Associations.” PLoS Comput Biol. (2016) Dec 9;12(12):e1005244. doi: 10.1371/journal.pcbi.1005244

Smeda, J.R., Schilmiller, A.L., Last, R.L. and Mutschler, M. 2016. Introgression of acylsugar chemistry QTL modifies the composition and structure of acylsugars produced by high-accumulating tomato lines. Molec. Breeding, 36:160. doi: 10.1007/s11032-016-0584-6.

Fan P, Moghe GD, Last RL. “Comparative Biochemistry and In Vitro Pathway Reconstruction as Powerful Partners in Studies of Metabolic Diversity.” Methods Enzymol. (2016) 576:1-17. doi:10.1016/bs.mie.2016.02.023

Schilmiller AL, Gilgallon K, Ghosh B, Jones AD, Last RL. “Acylsugar acylhydrolases: carboxylesterase catalyzed hydrolysis of acylsugars in tomato trichomes.” Plant Physiol. (2016) Jan 25. doi:10.1104/pp.15.01348

Fan P, Miller AM, Schilmiller AL, Liu X, Ofner I, Jones AD, Zamir D, Last RL. “In vitro reconstruction and analysis of evolutionary variation of the tomato acylsucrose metabolic network.” Proc Natl Acad Sci U S A. (2016) Jan 12;113(2):E239-48. doi:10.1073/pnas.1517930113

Liu J, Last RL. “MPH1 is a thylakoid membrane protein involved in protecting photosystem II from photodamage in land plants.” Plant Signal Behav. (2015) 10(10):e1076602. doi:10.1080/15592324.2015.1076602

Moghe GD, Last RL. “Something Old, Something New: Conserved Enzymes and the Evolution of Novelty in Plant Specialized Metabolism.” Plant Physiol. (2015) Nov;169(3):1512-23. doi:10.1104/pp.15.00994

Peng C, Uygun S, Shiu SH, Last RL. “The Impact of the Branched-Chain Ketoacid Dehydrogenase Complex on Amino Acid Homeostasis in Arabidopsis.” Plant Physiol. (2015) Nov;169(3):1807-20. doi:10.1104/pp.15.00461

Ning J, Moghe GD, Leong B, Kim J, Ofner I, Wang Z, Adams C, Jones AD, Zamir D, Last RL. “A Feedback-Insensitive Isopropylmalate Synthase Affects Acylsugar Composition in Cultivated and Wild Tomato.” Plant Physiol. (2015) Nov;169(3):1821-35. doi:10.1104/pp.15.00474

Schilmiller AL, Moghe GD, Fan P, Ghosh B, Ning J, Jones AD, Last RL. “Functionally divergent alleles and duplicated Loci encoding an acyltransferase contribute to acylsugar metabolite diversity in Solanum trichomes.” Plant Cell. (2015) Apr;27(4):1002-17. doi:10.1105/tpc.15.00087

Liu J, Last RL. “A land plant-specific thylakoid membrane protein contributes to photosystem II maintenance in Arabidopsis thaliana.” Plant J. (2015) Jun;82(5):731-43. doi:10.1111/tpj.12845

Fristedt R, Herdean A, Blaby-Haas CE, Mamedov F, Merchant SS, Last RL, Lundin B. “PHOTOSYSTEM II PROTEIN33, a protein conserved in the plastid lineage, is associated with the chloroplast thylakoid membrane and provides stability to photosystem II supercomplexes in Arabidopsis.” Plant Physiol. (2015) Feb;167(2):481-92. doi:10.1104/pp.114.253336


Kim J, Matsuba Y, Ning J, Schilmiller AL, Hammar D, Jones AD, Pichersky E, Last RL. “Analysis of Natural and Induced Variation in Tomato Glandular Trichome Flavonoids Identifies a Gene Not Present in the Reference Genome.” The Plant Cell (August 2014) doi:10.1105/tpc.114.129460

Kim J, Kang K, Gonzales-Vigil E, Shi E, Jones AD, Barry CS, Last RL. “Striking Natural Diversity in Glandular Trichome Acylsugar Composition Is Shaped by Variation at the Acyltransferase2 Locus in the Wild Tomato Solanum habrochaites.” PLANT PHYSIOLOGY 160.4 (2012): 1854-70. doi:10.1104/pp.112.204735.

Schilmiller AL, Charbonneau AL, Last RL. “Identification of a BAHD acetyltransferase that produces protective acyl sugars in tomato trichomes.” Proceedings of the National Academy of Sciences 109.40 (2012): 16377-82. doi:10.1073/pnas.1207906109.

Milo R, Last RL. “Achieving Diversity in the Face of Constraints: Lessons from Metabolism.” Science 336.6089 (2012): 1663-67. doi:10.1126/science.1217665.