Recent Publications

Hausinger RP, Desguin B, Fellner M, Rankin JA, Hu J. “Nickel-pincer nucleotide cofactor.” Curr Opin Chem Biol. (2018) Jul 9;47:18-23. doi: 10.1016/j.cbpa.2018.06.019

Li M, Henderson KL, Martinez S, Hausinger RP, Emerson JP. “The Irving-Williams series and the 2-His-1-carboxylate facial triad: a thermodynamic study of Mn2+, Fe2+, and Co2+ binding to taurine/α-ketoglutarate dioxygenase (TauD).” J Biol Inorg Chem. (2018) Jun 19;. doi: 10.1007/s00775-018-1574-4

Desguin B, Fellner M, Riant O, Hu J, Hausinger R, Hols P, Soumillion P. “Biosynthesis of the nickel-pincer nucleotide cofactor of lactate racemase requires a CTP-dependent cyclometallase.” J Biol Chem. (2018) Jun 10. doi: 10.1074/jbc.RA118.003741

Herr CQ, Hausinger RP. “Amazing Diversity in Biochemical Roles of Fe(II)/2-Oxoglutarate Oxygenases.” Trends Biochem Sci. (2018) Apr 27. doi: 10.1016/j.tibs.2018.04.002

Eschweiler JD, Farrugia MA, Dixit SM, Hausinger RP, Ruotolo BT. “A Structural Model of the Urease Activation Complex Derived from Ion Mobility-Mass Spectrometry and Integrative Modeling.” Structure. (2018) Apr 3;26(4):599-606.e3. doi: 10.1016/j.str.2018.03.001

Rankin JA, Mauban RC, Fellner M, Desguin B, McCracken J, Hu J, Varganov SA, Hausinger RP. “The Lactate Racemase Nickel-Pincer Cofactor Operates by a Proton-Coupled Hydride Transfer Mechanism.” Biochemistry. (2018) Feb 28. doi: 10.1021/acs.biochem.8b00100

Müller TA, Struble SL, Meek K, Hausinger RP. “Characterization of human AlkB homolog 1 produced in mammalian cells and demonstration of mitochondrial dysfunction in ALKBH1-deficient cells.” Biochem Biophys Res Commun. (2017) Oct 30. doi: 10.1016/j.bbrc.2017.10.158

Fellner M, Desguin B, Hausinger RP, Hu J. “Structural insights into the catalytic mechanism of a sacrificial sulfur insertase of the N-type ATP pyrophosphatase family, LarE.” Proc Natl Acad Sci U S A. (2017) Aug 7. pii: 201704967. doi: 10.1073/pnas.1704967114

Martinez S, Fellner M, Herr CQ, Ritchie A, Hu J, Hausinger RP. “Structures and Mechanisms of the Non-Heme Fe(II)- and 2-Oxoglutarate-Dependent Ethylene-Forming Enzyme: Substrate Binding Creates a Twist.” J Am Chem Soc. (2017) Aug 5. doi: 10.1021/jacs.7b06186

Desguin B, Soumillion P, Hausinger RP, Hols P. “Unexpected complexity in the lactate racemization system of lactic acid bacteria.” FEMS Microbiol Rev. (2017) Aug 1;41(Supp_1):S71-S83. doi: 10.1093/femsre/fux021

Martinez S, Hausinger RP. “Correction to Biochemical and Spectroscopic Characterization of the Non-Heme Fe(II)- and 2-Oxoglutarate-Dependent Ethylene-Forming Enzyme from Pseudomonas syringae pv. phaseolicola PK2.” Biochemistry. (2017) Jun 20;56(24):3158. doi: 10.1021/acs.biochem.7b00502

Müller TA, Tobar MA, Perian MN, Hausinger RP. “Biochemical characterization of AP lyase and m(6)A demethylase activities of human AlkB homolog 1 (ALKBH1).” Biochemistry. (2017) Mar 14. doi: 10.1021/acs.biochem.7b00060

Walker AR, Silvestrov P, Müller TA, Podolsky RH, Dyson G, Hausinger RP, Cisneros GA. “ALKBH7 Variant Related to Prostate Cancer Exhibits Altered Substrate Binding.” PLoS Comput Biol. (2017) Feb 23;13(2):e1005345. doi: 10.1371/journal.pcbi.1005345

Henderson KL, Li M, Martinez S, Lewis EA, Hausinger RP, Emerson JP. “Global stability of an α-ketoglutarate-dependent dioxygenase (TauD) and its related complexes.” Biochim Biophys Acta. (2017) Feb 15. pii: S0304-4165(17)30066-1. doi: 10.1016/j.bbagen.2017.02.018

Masukawa H, Sakurai H, Hausinger RP, Inoue K. “Increased heterocyst frequency by patN disruption in Anabaena leads to enhanced photobiological hydrogen production at high light intensity and high cell density.” Appl Microbiol Biotechnol. (2017) Jan 7. doi: 10.1007/s00253-016-8078-3

Martin-Diaconescu V, Joseph CA, Boer JL, Mulrooney SB, Hausinger RP, Maroney MJ. “Non-thiolate ligation of nickel by nucleotide-free UreG of Klebsiella aerogenes.” J Biol Inorg Chem. (2016) Dec 21. doi: 10.1007/s00775-016-1429-9

Proshlyakov DA, McCracken J, Hausinger RP. “Spectroscopic analyses of 2-oxoglutarate-dependent oxygenases: TauD as a case study.” J Biol Inorg Chem. (2016) Nov 3;. doi: 10.1007/s00775-016-1406-3

Martinez S, Hausinger RP. “Biochemical and Spectroscopic Characterization of the Non-Heme Fe(II) and 2-Oxoglutarate Dependent Ethylene-Forming Enzyme from Pseudomonas syringae pv. phaseolicola PK2.” Biochemistry. (2016) Oct 17. doi: 10.1021/acs.biochem.6b00890

Desguin B, Soumillion P, Hols P, Hausinger RP. “Nickel-pincer cofactor biosynthesis involves LarB-catalyzed pyridinium carboxylation and LarE-dependent sacrificial sulfur insertion.” Proc Natl Acad Sci U S A. (2016) May 17;113(20):5598-603. doi:10.1073/pnas.1600486113

Farrugia MA, Wang B, Feig M, Hausinger RP. “Mutational and Computational Evidence That a Nickel-Transfer Tunnel in UreD Is Used for Activation of Klebsiella aerogenes Urease.” Biochemistry. (2015) Oct 20;54(41):6392-401. doi:10.1021/acs.biochem.5b00942

Martinez S, Hausinger RP. “Catalytic Mechanisms of Fe(II)- and 2-Oxoglutarate-dependent Oxygenases.” J Biol Chem. (2015) Aug 21;290(34):20702-11. doi:10.1074/jbc.R115.648691

Desguin B, Zhang T, Soumillion P, Hols P, Hu J, Hausinger RP. “METALLOPROTEINS. A tethered niacin-derived pincer complex with a nickel-carbon bond in lactate racemase.” Science. (2015) Jul 3;349(6243):66-9. doi:10.1126/science.aab2272

Henderson KL, Müller TA, Hausinger RP, Emerson JP. “Calorimetric assessment of Fe(2+) binding to α-ketoglutarate/taurine dioxygenase: ironing out the energetics of metal coordination by the 2-His-1-carboxylate facial triad.” Inorg Chem. (2015) Mar 2;54(5):2278-83. doi:10.1021/ic502881q

Macomber L, Minkara MS, Hausinger RP, Merz KM Jr. “Reduction of urease activity by interaction with the flap covering the active site.” J Chem Inf Model. (2015) Feb 23;55(2):354-61. doi:10.1021/ci500562t

Silvestrov P, Müller TA, Clark KN, Hausinger RP, Cisneros GA. “Homology modeling, molecular dynamics, and site-directed mutagenesis study of AlkB human homolog 1 (ALKBH1).” J Mol Graph Model. (2014) Nov;54:123-30. doi:10.1016/j.jmgm.2014.10.013

Yu H, Hausinger RP, Tang HZ, Xu P. “Mechanism of the 6-hydroxy-3-succinoyl-pyridine 3-monooxygenase flavoprotein from Pseudomonas putida S16.” J Biol Chem. (2014) Oct 17;289(42):29158-70. doi:10.1074/jbc.M114.558049

Boer JL, Mulrooney SB, Hausinger RP. “Nickel-dependent metalloenzymes.” Arch Biochem Biophys. (2014) Feb 15;544:142-52. doi:10.1016/

Casey TM, Grzyska PK, Hausinger RP, McCracken J. “Measuring the orientation of taurine in the active site of the non-heme Fe(II)/α-ketoglutarate-dependent taurine hydroxylase (TauD) using electron spin echo envelope modulation (ESEEM) spectroscopy.” J Phys Chem B. (2013) Sep 12;117(36):10384-94. doi:10.1021/jp404743d

Müller TA, Yu K, Hausinger RP, Meek K. “ALKBH1 is dispensable for abasic site cleavage during base excision repair and class switch recombination.” PLoS One. (2013);8(6):e67403. doi:10.1371/journal.pone.0067403

Farrugia MA, Han L, Zhong Y, Boer JL, Ruotolo BT, Hausinger RP. “Analysis of a soluble (UreD:UreF:UreG)2 accessory protein complex and its interactions with Klebsiella aerogenes urease by mass spectrometry.” J Am Soc Mass Spectrom. (2013) Sep;24(9):1328-37. doi:10.1007/s13361-013-0677-y

Müller TA, Andrzejak MM, Hausinger RP. “A covalent protein-DNA 5'-product adduct is generated following AP lyase activity of human ALKBH1 (AlkB homologue 1).” Biochem J. (2013) Jun 15;452(3):509-18. doi:10.1042/BJ20121908

Farrugia MA, Macomber L, Hausinger RP. “Biosynthesis of the urease metallocenter.” J Biol Chem. (2013) May 10;288(19):13178-85. doi:10.1074/jbc.R112.446526

Masukawa H, Kitashima M, Inoue K, Sakurai H, Hausinger RP. “Genetic engineering of cyanobacteria to enhance biohydrogen production from sunlight and water.” Ambio. (2012);41 Suppl 2:169-73. doi:10.1007/s13280-012-0275-4

Simmons JM, Koslowsky DJ, Hausinger RP. “Characterization of a Trypanosoma brucei Alkb homolog capable of repairing alkylated DNA.” Exp Parasitol. (2012) May;131(1):92-100. doi:10.1016/j.exppara.2012.03.011

Boer JL, Hausinger RP. “Klebsiella aerogenes UreF: identification of the UreG binding site and role in enhancing the fidelity of urease activation.” Biochemistry. (2012) Mar 20;51(11):2298-308. doi:10.1021/bi3000897