BMB 472 Experimental Biochemistry
Growth of bacteria monitored by spectrophotometry.
Growth of bacteriophage, titering, and transduction.
Preparation of bacteriophage and plasmid DNA.
Electrophoretic Analysis of DNA preps.
Directional cloning of topAcysB into pTrc99A.
Transformation of DM700 and XL-1 Blue with pTrc99A/topAcysB
Electrophoretic Analysis of Restricted DNAs and Products of Ligation
PCR Amplification of DNA
Analysis of Topoisomerase I Assays
Ligation of topA DNA into M13mp19
Densitometric Analysis of SDS-PAGE Gels and Western Blots
Transfection of F' E. coli cells with the Ligation Mixture of topA DNA Inserted into M13mp19
Orientation Analysis of topA Subclones by a Complimentarity Test