BMB 472 Experimental Biochemistry
- Experiment 2A
Growth of bacteria monitored by spectrophotometry.
- Experiment 2B
Growth of bacteriophage, titering, and transduction.
- Experiments 3A & 3B
Preparation of bacteriophage and plasmid DNA.
- Experiment 3C
Electrophoretic Analysis of DNA preps.
- Experiment 4B
Directional cloning of topAcysB into pTrc99A.
- Experiment 5A
Transformation of DM700 and XL-1 Blue with pTrc99A/topAcysB
- Experiment 5B
Electrophoretic Analysis of Restricted DNAs and Products of Ligation
- Experiment 9A
PCR Amplification of DNA
- Experiment 9B
Analysis of Topoisomerase I Assays
- Experiment 10B
Ligation of topA DNA into M13mp19
- Experiment 11B
Densitometric Analysis of SDS-PAGE Gels and Western Blots
- Experiment 11C
Transfection of F' E. coli cells with the Ligation Mixture of topA DNA Inserted into M13mp19
- Experiment 13
Orientation Analysis of topA Subclones by a Complimentarity Test