BMB 472 Experimental Biochemistry
Experimental Approach to Molecular Biochemistry
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BMB 472 Learning Modules
- Experiment 2A
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Growth of bacteria monitored by spectrophotometry.
- Experiment 2B
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Growth of bacteriophage, titering, and transduction.
- Experiments 3A & 3B
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Preparation of bacteriophage and plasmid DNA.
- Experiment 3C
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Electrophoretic Analysis of DNA preps.
- Experiment 4B
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Directional cloning of topAcysB into pTrc99A.
- Experiment 5A
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Transformation of DM700 and XL-1 Blue with pTrc99A/topAcysB
- Experiment 5B
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Electrophoretic Analysis of Restricted DNAs and Products of Ligation
- Experiment 9A
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PCR Amplification of DNA
- Experiment 9B
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Analysis of Topoisomerase I Assays
- Experiment 10B
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Ligation of topA DNA into M13mp19
- Experiment 11B
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Densitometric Analysis of SDS-PAGE Gels and Western Blots
- Experiment 11C
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Transfection of F' E. coli cells with the Ligation Mixture of topA DNA Inserted into M13mp19
- Experiment 13
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Orientation Analysis of topA Subclones by a Complimentarity Test