BMB 472 Experimental Biochemistry

Experimental Approach to Molecular Biochemistry

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BMB 472 Learning Modules

Experiment 2A

Growth of bacteria monitored by spectrophotometry.

Experiment 2B

Growth of bacteriophage, titering, and transduction.

Experiments 3A & 3B

Preparation of bacteriophage and plasmid DNA.

Experiment 3C

Electrophoretic Analysis of DNA preps.

Experiment 4B

Directional cloning of topAcysB into pTrc99A.

Experiment 5A

Transformation of DM700 and XL-1 Blue with pTrc99A/topAcysB

Experiment 5B

Electrophoretic Analysis of Restricted DNAs and Products of Ligation

Experiment 9A

PCR Amplification of DNA

Experiment 9B

Analysis of Topoisomerase I Assays

Experiment 10B

Ligation of topA DNA into M13mp19

Experiment 11B

Densitometric Analysis of SDS-PAGE Gels and Western Blots

Experiment 11C

Transfection of F' E. coli cells with the Ligation Mixture of topA DNA Inserted into M13mp19

Experiment 13

Orientation Analysis of topA Subclones by a Complimentarity Test