Directional cloning of the topAcysB fragment into pTrc99A

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We want to subclone the topAcysB insert from lambdaZAPII/topAcysB into the expression vector pTrc99A

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The cloning strategy is as follows:

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SacI and XbaI have different recognition sites, and leave incompatable ends

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The insert can only go into the vector in one direction

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We will analyze the success of this cloning experiment by using agarose gel electrophoresis in Experiment 5B