Learning Module - Experiment 4B
Directional cloning of the topAcysB fragment into pTrc99A
We want to subclone the topAcysB insert from lambdaZAPII/topAcysB into the expression vector pTrc99A
The cloning strategy is as follows:
SacI and XbaI have different recognition sites, and leave incompatable ends
The insert can only go into the vector in one direction
We will analyze the success of this cloning experiment by using agarose gel electrophoresis in Experiment 5B