Recent Publications
Rivett, E.D.; Addis, H.G.; Dietz, J.V.; Carroll-Deaton, J.A.; Gupta, S.; Foreman, K.L.; Dang, M.A.; Fox, J.L.*; Khalimonchuk, O.*; Hegg, E.L.* “Evidence that the catalytic mechanism of heme a synthase involves the formation of a carbocation stabilized by a conserved glutamate.” Arch. Biochem. Biophys. 2023, 744, e109665. doi: 10.1016/j.abb.2023.109665
Fang, Z.; Jackson, J.E.*; Hegg, E.L.* “Mild, electroreductive lignin cleavage: Optimizing the depolymerization of authentic lignins.” ACS Sustainable Chem. Eng. 2022, 10, 7545-7552. doi: 10.1021/acssuschemeng.2c00820
Zhou, Y.; Klinger, G.E.; Hegg, E.L.; Saffron, C.M.; Jackson, J.E.* “Skeletal Ni Electrode-Catalyzed C-O Cleavage of Diaryl Ethers Entails Direct Elimination via Benzyne Intermediates.” Nat. Commun. 2022, 13, e2050. doi: 10.1038/s41467-022-29555-3
Yuan, Z.; Bals, B.D.*; Hegg, E.L.*; Hodge, D.B.* “Technoeconomic evaluation of recent process improvements in production of sugar and high-value lignin co-products via two-stage Cu-catalyzed alkaline-oxidative pretreatment.” Biotechnol. Biofuels Bioprod. 2022, 15, e45. doi: 10.1186/s13068-022-02139-5
Chin, M.; Suh, S.M.; Fang, Z.; Hegg, E.L.; Diao, T.* “Depolymerization of Lignin via a Microscopic Reverse Biosynthesis Pathway.” ACS Catal. 2022, 12, 2532-2539. doi: 10.1021/acscatal.2c00133
Alherech, M.; Omolabake, S.; Holland, C.M.; Klinger, G.E.; Hegg, E.L.; Stahl, S.S.* “From lignin to valuable aromatic chemicals: Lignin depolymerization and monomer separation via centrifugal partition chromatography.” ACS Cent. Sci. 2021, 7, 1831-1837. doi: 10.1021/acscentsci.1c00729
Rivett, E.D.; Heo, L.; Feig, M.; Hegg, E.L.* “Biosynthesis and trafficking of heme o and heme a: new structural insights and their implications for reaction mechanisms and prenylated heme transfer.” Crit. Rev. Biochem. Mol. Biol. 2021, 56, 640-668. doi: 10.1080/10409238.2021.1957668
Sosa Alfaro, V.; Campeciño, J.; Tracy, M.; Elliott, S.J.*; Hegg, E.L.*; Lehnert, N.* “Elucidating Electron Storage and Distribution within the Pentaheme Scaffold of Cytochrome c Nitrite Reductase (NrfA).” Biochemistry 2021, 60, 1853-1867. doi: 10.1021/acs.biochem.0c00977
Fang, Z.; Flynn, M.G.; Jackson, J.E.*; Hegg, E.L.* “Thio-assisted reductive electrolytic cleavage of lignin β-O-4 models and authentic lignin.” Green Chem. 2021, 23, 412-421. doi: 10.1039/D0GC03597A
Yuan, Z.; Klinger, G.E.; Nikafshar, S.; Cui, Y.; Alherech, M.; Goes, S.; Anson, C.; Singh, S.K.; Hodge, D.B.*; Bals, B.*; Nejad, M.*; Stahl, S.S.*; Hegg, E.L.* "Effective biomass fractionation through oxygen-enhanced alkaline–oxidative pretreatment." ACS Sustainable Chem. Eng. 2021, 9, 1118-1127. doi: 10.1021/acssuschemeng.0c06170
Klinger, G.E.; Zhou, Y.; Foote, J.A.; Wester, A.M.; Cui, Y.; Alherech, M.; Stahl, S.S.; Jackson, J.E.*; Hegg, E.L.* "Nucleophilic Thiols Reductively Cleave Ether Linkages in Lignin Model Polymers and Lignin." ChemSusChem 2020, 13, 4394-4399.
Campeciño, J.; Lagishetty, S.; Wawrzak, Z.; Sosa Alfaro, V.; Lehnert, N.; Reguera, G.; Hu, J.; Hegg, E.L.* "Cytochrome c nitrite reductase from the bacterium Geobacter lovleyi represents a new NrfA subclass." J. Biol. Chem. 2020, 295, 11455-11465.
Fang, Z.*; Nikafshar, S.; Hegg, E.L.; Nejad, M.* "Biobased Divanillin As a Precursor for Formulating Biobased Epoxy Resin." ACS Sustainable Chem. Eng. 2020, 8, 9095-9103.
Zhou, Y.; Klinger, G.E.; Hegg, E.L.; Saffron, C.M.; Jackson, J.E.* “Multiple Mechanisms Mapped in Aryl Alkyl Ether Cleavage via Aqueous Electrocatalytic Hydrogenation (ECH) over Skeletal Nickel.” J. Am. Chem. Soc. 2020, 142, 4037-4050.
Srivastava1, P.A.; Hegg, E.L.; Fox, B.G.; Yennamalli, R.M.* “PreDSLpmo: A Neural Network-Based Prediction Tool for Functional Annotation of Lytic Polysaccharide Monooxygenases.” J. Biotechnol. 2020, 308, 148-155.
Bhalla, A.; Cai, C.; Xu, F.; Singh, S.K.; Bansal, N.; Phongpreecha, T.; Dutta, T.; Foster, C.; Kumar, R.; Simmons, B.; Singh, S.; Wyman, C.E.; Hegg, E.L.*; Hodge, D.B.* “Performance of three delignifying pretreatments on hardwoods: hydrolysis yields, comprehensive mass balances, and lignin properties.” Biotech. Biofuels 2019, 12, e213. doi: 10.1186/s13068-019-1546-0
Klinger, G.E.; Zhou, Y.; Hao, P.; Robbins, J.; Aquilina, J.; Foster, C.; Jackson, J.E.*; Hegg, E.L.* “Biomimetic Reductive Cleavage of Keto Aryl Ether Bonds by Small Molecule Thiols.” ChemSusChem 2019, 12, 4775-4779. doi: 10.1002/cssc.201901742
Singh, S.K.; Savoy, A.W.; Yuan, Z.; Hao, L.; Stahl, S.S.; Hegg, E.L.*; Hodge, D.B.* “Integrated Two-Stage Alkaline-Oxidative Pretreatment of Hybrid Poplar. Part 1: Impact of Alkaline Pre-Extraction Conditions on Process Performance and Lignin Properties.” Ind. Eng. Chem. Res. 2019, 58, 15989-15999. doi: 10.1021/acs.iecr.9b01124
Yuan, Z.; Singh, S.K.; Bals, B.; Hodge, D.B.*; Hegg, E.L.* “Integrated Two-Stage Alkaline–Oxidative Pretreatment of Hybrid Poplar. Part 2: Impact of Cu-Catalyzed Alkaline Hydrogen Peroxide Pretreatment Conditions on Process Performance and Economics.” Ind. Eng. Chem. Res. 2019, 58, 16000-16008. doi: 10.1021/acs.iecr.9b00901
Herwaldt, E.J.; White, A.; Rivett, E.D. Hegg, E.L.* “Cox15 interacts with the cytochrome bc1 dimer within respiratory supercomplexes as well as in the absence of cytochrome c oxidase.” J. Biol Chem. 2018, 293, 16426-16439. doi: 10.1074/jbc.RA118.002496
Yuan, Z.; Li, G.;* Hegg, E.L. “Enhancement of sugar recovery and ethanol production from wheat straw through alkaline pre-extraction followed by steam pretreatment.” Bioresource Technol. 2018, 266, 194-202. doi: 10.1016/j.biortech.2018.06.065
Bhalla, A.; Fasahati, P.; Particka, C.A.; Assad, A.E.; Stoklosa, R.J.; Bansal, N.; Semaan, R.; Saffron, C.M.*; Hodge, D.B.*; Hegg, E.L.* “Integrated experimental and technoeconomic evaluation of two-stage Cu-catalyzed alkaline-oxidative pretreatment of hybrid poplar.” Biotechnol. Biofuels 2018, 11, e143. doi: 10.1186/s13068-018-1124-x
Haslun, J.A.; Ostrom, N.E.*; Hegg, E.L.; Ostrom, P.H.* “Estimation of Isotope Variation of N2O during Denitrification by Pseudomonas aureofaciens and Pseudomonas chlororaphis: Implications for N2O Source Apportionment.” Biogeosciences. 2018, 15, 3873-3882. doi: 10.5194/bg-15-3873-2018
Das, A.; Rahimi, A.; Ulbrich, A.; Alherech, M.; Motagamwala, A.H.; Bhalla, A. da Costa Sousa, L.; Balan, V.; Dumesic, J.A.; Hegg, E.L.; Dale, B.E.; Ralph, J.; Coon, J.J.*; Stahl, S.S.* ”Lignin Conversion to Low-Molecular-Weight Aromatics via an Aerobic Oxidation-Hydrolysis Sequence: Comparison of Different Lignin Sources." ACS Sustainable Chem. Eng. 2018, 6, 3367-3374. (Selected for the virtual special issue Festschrift in Honor of István T. Horváth.) doi: 10.1021/acssuschemeng.7b03541
Bhalla, A.; Bansal, N.; Pattathil, S.; Li, M.; Shen, W.; Particka, C.A.; Karlen, S.D.; Phongpreecha, T.; Semaan, R.; Gonzales-Vigil, E.; Ralph, J.; Mansfield, S.D.; Ding, S-Y.; Hodge, D.B.*; Hegg, E.L.* ”Engineered Lignin in Poplar Biomass Facilitates Cu-Catalyzed Alkaline-Oxidative Pretreatment." ACS Sustainable Chem. Eng. 2018, 6, 2932-2941. doi: 10.1021/acssuschemeng.7b02067
Cornish, A.J.; Ginovska, B.; Thelen, A.; da Silva, J.C.S.; Soares, T.A.; Raugei, S.; Dupuis, M.; Shaw, W.J.*; Hegg, E.L.* “Single-Amino Acid Modifications Reveal Additional Controls on the Proton Pathway of [FeFe]-Hydrogenase.” Biochemistry. 2016, 55, 3165-3173. doi: 10.1021/acs.biochem.5b01044
Bhalla, A.; Bansal, N.; Stoklosa, R.J.; Fountain, M.; Ralph, J.; Hodge, D.B.*; Hegg, E.L.* “Effective alkaline metal-catalyzed oxidative delignification of hybrid poplar.” Biotechnol Biofuels. 2016, 9, e34. doi: 10.1186/s13068-016-0442-0
Aussignargues, C.; Pandelia, M.E.; Sutter, M.; Plegaria, J.S.; Zarzycki, J.; Turmo, A.; Huang, J.; Ducat, D.C.; Hegg, E.L.; Gibney, B.R.; Kerfeld, C.A.* “Structure and Function of a Bacterial Microcompartment Shell Protein Engineered to Bind a [4Fe-4S] Cluster.” J. Am. Chem. Soc. 2016, 138, 5262-5270.
Yang, H.; Gandhi, H.; Moran, J.J.; Kreuzer, H.W.; Ostrom, N.E.*; Hegg, E.L.* “Isotopic Fractionation Associated with [NiFe]- and [FeFe]-Hydrogenases.” Rapid Commun. Mass. Spectrom. 2016, 30, 285-292. doi: 10.1002/rcm.7432
Bansal, N.; Bhalla, A.; Pattathil, S.; Adelman, S.L.; Hahn, M.G.; Hodge, D.B.*; Hegg, E.L.* “Cell Wall-Associated Transition Metals Improve Alkaline-Oxidative Pretreatment in Diverse Hardwoods.” Green Chem. 2016, 18, 1405-1415. doi: 10.1039/C5GC01748C
Aussignargues, C.; Pandelia, M.E.; Sutter, M.; Plegaria, J.S.; Zarzycki, J.; Turmo, A.; Huang, J.; Ducat, D.C.; Hegg, E.L.; Gibney, B.R.; Kerfeld, C.A.* “Structure and Function of a Bacterial Microcompartment Shell Protein Engineered to Bind a [4Fe-4S] Cluster.” J Am Chem Soc. (2016) Apr 27;138(16):5262-70. doi: 10.1021/jacs.5b11734
Li Z., Bansal N., Azarpira A., Bhalla A., Chen C.H., Ralph J., Hegg E.L., Hodge D.B. “Chemical and structural changes associated with Cu-catalyzed alkaline-oxidative delignification of hybrid poplar.” Biotechnol Biofuels. (2015);8:123. doi: 10.1186/s13068-015-0300-5
Cornish A.J., Green R., Gärtner K., Mason S., Hegg E.L. “Characterization of Hydrogen Metabolism in the Multicellular Green Alga Volvox carteri.” PLoS One. (2015);10(4):e0125324. doi: 10.1371/journal.pone.0125324
Yang H., Gandhi H., Ostrom N.E., Hegg E.L. “Isotopic fractionation by a fungal P450 nitric oxide reductase during the production of N2O.” Environ Sci Technol. (2014) Sep 16;48(18):10707-15. doi: 10.1021/es501912d
Quaroni L., Zlateva T., Sarafimov B., Kreuzer H.W., Wehbe K., Hegg E.L., Cinque G. “Synchrotron based infrared imaging and spectroscopy via focal plane array on live fibroblasts in D2O enriched medium.” Biophys Chem. (2014) May;189:40-8. doi: 10.1016/j.bpc.2014.03.002
Hildenbeutel M., Hegg E.L., Stephan K., Gruschke S., Meunier B., Ott M. “Assembly factors monitor sequential hemylation of cytochrome b to regulate mitochondrial translation.” J Cell Biol. (2014) May 26;205(4):511-24. doi: 10.1083/jcb.201401009
Kreuzer H.W., Hill E.A., Moran J.J., Bartholomew R.A., Yang H., Hegg E.L. “Contributions of the [NiFe]- and [FeFe]-hydrogenase to H2 production in Shewanella oneidensis MR-1 as revealed by isotope ratio analysis of evolved H(2).” FEMS Microbiol Lett. (2014) Mar;352(1):18-24. doi: 10.1111/1574-6968.12361
Li Z., Chen C.H., Hegg E.L., Hodge D.B. “Rapid and effective oxidative pretreatment of woody biomass at mild reaction conditions and low oxidant loadings.” Biotechnol Biofuels. (2013) Aug 26;6(1):119. doi: 10.1186/1754-6834-6-119
MacGregor B.J., Biddle J.F., Siebert J.R., Staunton E., Hegg E.L., Matthysse A.G., Teske A. “Why orange Guaymas Basin Beggiatoa spp. are orange: single-filament-genome-enabled identification of an abundant octaheme cytochrome with hydroxylamine oxidase, hydrazine oxidase, and nitrite reductase activities.” Appl Environ Microbiol. (2013) Feb;79(4):1183-90. doi: 10.1128/AEM.02538-12
Li Z., Chen C.H., Liu T., Mathrubootham V., Hegg E.L., Hodge D.B. “Catalysis with Cu(II) (bpy) improves alkaline hydrogen peroxide pretreatment.” Biotechnol Bioeng. (2013) Apr;110(4):1078-86. doi: 10.1002/bit.24793
Vieler A., Wu G., Tsai C.H., Bullard B., Cornish A.J., Harvey C., Reca I.B., Thornburg C., Achawanantakun R., Buehl C.J., Campbell M.S., Cavalier D., Childs K.L., Clark T.J., Deshpande R., Erickson E., Armenia Ferguson A., Handee W., Kong Q., Li X., Liu B., Lundback S., Peng C., Roston R.L., Sanjaya, Simpson J.P., Terbush A., Warakanont J., Zäuner S., Farre E.M., Hegg E.L., Jiang N., Kuo M.H., Lu Y., Niyogi K.K., Ohlrogge J., Osteryoung K.W., Shachar-Hill Y., Sears B.B., Sun Y., Takahashi H., Yandell M., Shiu S.H., Benning C. “Genome, functional gene annotation, and nuclear transformation of the heterokont oleaginous alga Nannochloropsis oceanica CCMP1779.” PLoS Genet. (2012);8(11):e1003064. doi: 10.1371/journal.pgen.1003064
Gärtner K., Lechno-Yossef S., Cornish A.J., Wolk C.P., Hegg E.L. “Expression of Shewanella oneidensis MR-1 [FeFe]-hydrogenase genes in Anabaena sp. strain PCC 7120.” Appl Environ Microbiol. (2012) Dec;78(24):8579-86. doi: 10.1128/AEM.01959-12