Protocols

1. Affinity purification
2. Agrobacterium Transformation and Competent Cell Preparation
3. Antibody labelling
4. Arabidopsis FW, DW to Area Conversion
5. Bacterial Transformation and Plasmid Prep
6. Bradford Assay
7. Calculating Isoprene in Liquid Phase
8. Calculating Plant Isoprene from FIS Data
9. Calculating Total Isoprene In Gas and Liquid Phase
10. Carbo Assay Plate Reader
11. Carbo Assay Spec
12. Chemical fixation
13. Competent Cell generation and transformation
14. Denaturing Reduced Protein Gels, Coomassie Staining, and Western Blot
15. DMADP assay
16. DNA Extraction (Qiagen Kit)
17. DNA Extraction
18. DNA Gels
19. DNA or PCR Cleanup Kit Promega
20. DNase Treatment using RQ1 (Promega)
21. DXP Assay
22. E. coli Filter Paper Blots for Shipping Strains
23. Estimating Bacterial Cytosol Volume based on OD, Dry Weight or Wet Weight
24. Flow meter calibration
25. Freeze drying
26. Gas exchange
27. Glass knives
28. Growth Media
29. H₂O₂Assay (Amplex Red)
30. His-tagged Protein Purification with Protease Inhibitor
31. Ion exchange chromatography
32. kcat
33. Kramer spec -determination of deconvolution formula
34. LB Plates and Liquid
35. Lipid Extraction
36. Lowry Protein Assay
37. Malate Dehydrogenase Assay
38. Mass spectrometry
39. Microwave-assisted sample preparation for TEM
40. Miracle-Gro Instructions
41. PCR High Fidelity
42. PCR
43. PEPCase Assay without Radioactivity (Cytosol Marker)
44. Perchloric acid extraction
45. Phosphate Assay for DMADP Quantification
46. Potting Arabidopsis
47. Potting poplar
48. Primer Design with Primer 3
49. Protein Extraction from Plant Material for Lowry Assay
50. qPCR
51. Restriction Digest, Dephosphorylation, Gel Purification, Ligation
52. RNA Extraction and cDNA Synthesis
53. RNA Extraction using off column DNase Treatment
54. SALK or SAIL tDNA insert lines
55. Sandwich ELISA protocol -need optimization
56. Sectioning
57. Selecting Homozygous Transformed Plants
58. Sowing Arabidopsis Seeds
59. Staining grid with uranyl acetate and lead citrate for TEM
60. TEM
61. Transforming E.Coli, isolating plasmid and making glycerol stocks
62. Two-wavelength spec
63. Western